Faculty of Engineering and Natural Sciences · Molecular Biology and Genetics (English 30%) · Undergraduate
Course Objective
The aim of this course is to provide students with a comprehensive foundation in the principles and applications of recombinant DNA technology.
Course Content
This course introduces the fundamental principles and applications of recombinant DNA technology. Students will learn the key tools and techniques of molecular cloning, sequencing, and genome editing, with applications in medicine, agriculture, and industry. The course also explores ethical and biosafety considerations.
Required Resources
Gene Cloning and DNA Analysis, 8th Ed., TA Brown, 2021 John Wiley & Sons Ltd.
Recommended Resources
1. Principles of Gene Manipulation, 6th Ed., Sandy B. Primrose, Richard M Twyman & Robert W Old.
2. Molecular biotechnology: principles and applications of recombinant DNA, 4th Ed., Bernard R. Glick, Jack J. Pasternak, and Cheryl L. Patten.
Explanations
1. A midterm exam will be held during the semester, and a comprehensive final exam will be held at the end of the semester.
2. Three of four short quizzes and one or two assignments (not exceeding five in total) will be given during the semester.
3. The dates for the short quizzes and assignments will be announced to students during classes.
Rules
Students are required to comply with and follow the rules and ethical principles regarding course attendance, exams, and assignments as specified in the IZU regulations.
Core Area Distribution
Teaching Methods
Assessment & Evaluation
ECTS / Workload
| Activity | Quantity | Duration (h) | Total Workload |
|---|---|---|---|
| Course Duration (Including Exam Week) | 15 | 3 | 45 |
| Out of Class Study Period | 15 | 2 | 30 |
| Midterm | 1 | 10 | 10 |
| Quiz | 4 | 5 | 20 |
| Assignment | 1 | 5 | 5 |
| Practice | 0 | 0 | 0 |
| Final | 1 | 15 | 15 |
Course Schedule
| Week | Subject | Preparation |
|---|---|---|
| 1 | Introduction to Recombinant DNA Technology | Course materials will be shared |
| 2 | DNA Restriction Enzymes | Course materials will be shared |
| 3 | Cloning and Expression Vectors | Course materials will be shared |
| 4 | Gene transfer methods | Course materials will be shared |
| 5 | Identification of the generated recombinant cells and development of recombinant clones. | Course materials will be shared |
| 6 | The use of functional completion tests in the identification of recombinant clones | Course materials will be shared |
| 7 | Midterm | Midterm |
| 8 | Techniques to determine the gene locations | Course materials will be shared |
| 9 | Southern, Northern, Western and dot-blot analyses | Course materials will be shared |
| 10 | DNA sequence analysis and gene libraries | Course materials will be shared |
| 11 | Polymerase chain reaction and its usage in molecular diagnosis | Course materials will be shared |
| 12 | Recombinant protein production | Course materials will be shared |
| 13 | Vaccine and hormone production in microorganisms | Course materials will be shared |
| 14 | Transgenic plants and animals and their usage areas | Course materials will be shared |
| 15 | Revision | Revision |
| 16 | Final | Final |