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MBG 409 - Genomic and Proteomics

Faculty of Engineering and Natural Sciences · Molecular Biology and Genetics (English 30%) · Undergraduate

ECTS: 5 T+P+L: 3+0+0 Compulsory
Coordinator: Dr. Öğr. Üyesi Zeynep AYDIN SİNİRLİOĞLU

Course Objective

The aim of this course is for students to learn the concepts of genome, genome sequencing, genome expression, structural and functional genomes, proteome studies, and how to perform function and analysis of proteins.

Course Content

Genome definition, genome sequencing, genome anatomy, structural and functional analysis of the genome. The scope of proteomics includes: protein separation and identification methods, protein quantification methods, protein sequence analysis; protein structure and function.

Required Resources

T.A. Brown,Genomes 4, Taylor &Francis,Garland Science, (2023)

Twyman, R., Cfe, P.D., & George A., Garland Principles of Proteomics (2nd ed.).Science. (2013).

Explanations

A midterm, a final, and two short quizzes will be administered.
• Attendance will be governed by the exam regulations set by the dean's office. 
• The final exam topics will cover all subjects but will primarily focus on topics covered after the midterm.
• All exams will be in a traditional format and will include interpretive questions.

Rules

According to the regulations, a student who misses 30% of the total class hours (18 hours in total) will receive an absence (DZ) and fail the course. Attendance will be recorded with a signature.
Late Arrival: It's not just about arriving in class, but about joining the lesson on time. Remember that being late for three classes will count as one full absence. Late arrival is defined as: joining the class after the instructor has taken attendance and after the first 10 minutes have passed.
Seeking Help: Asking questions about the subject matter during class or in-person sessions is important for your education so you can learn the material in a timely manner. We encourage our students to seek help when needed.
Academic integrity: All work submitted for course grades is expected to be your own original work. Plagiarism or copying will result in disciplinary action and penalties in accordance with IZÜ's relevant regulations. You must provide medical reports if you are ill.
The assessments outlined in this program are fixed, and no additional compensatory assessments will be conducted to improve grades.

Course Learning Outcomes

  1. The student acquires basic knowledge about the genome and proteome.
  2. The student discusses the mechanisms involved in genome sequencing.
  3. Students gain fundamental knowledge about the structural and functional analysis of the genome.
  4. Students learn about protein separation, identification, and quantification.
  5. Students discuss protein sequence analysis, protein structure, and protein function.

Core Area Distribution

(44) Physical Sciences%60 (52) Engineering and Engineering Trades%40

Teaching Methods

ExpressionQuestion-AnswerDiscussion

Assessment & Evaluation

Performance Assignment ( Lab / Workshop / Field Work / Seminar / Presentation / Completion Study / ThesisTesting (Essay / Tests: True-Falls, multiple-choice, short answer, matching)

ECTS / Workload

ActivityQuantityDuration (h)Total Workload
Course Duration (Including Exam Week)16348
Out of Class Study Period16348
Midterm122
Quiz000
Assignment21326
Practice000
Final122

Course Schedule

WeekSubjectPreparation
1Introduction to genomicsTextbook
2Gene and genome structureTextbook
3Genome mappingTextbook
4Genome sequencing technologiesTextbook
5Genome annotationTextbook
6Functional and comparative genomicsTextbook
7TranscriptomicsTextbook
8Midterm examMidterm exam
9Introduction to ProteomicsTextbook
10Protein separation and characterization methodsTextbook
11Methods for determining protein contentTextbook
12Protein structure and sequence analysis methodsTextbook
13Protein-protein / protein-DNA interaction methodsTextbook
14Omics applications 1Textbook
15Omics applications 2Textbook
16FinalFinal